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Assistant Professor Dr.

Jeeraphong Thanongsaksrikul

Specialist: Immunology / Virology / Molecular biology / Antibody technology
Department: Graduated Program
Phone: +66911196867

Current Project

Project entitled “Production of truncated recombinant proteins of structural proteins of enterovirus 71 and coxsackievirus A16 for applying in immunological study”. Faculty of allied health sciences fund grant, Thammasat University (2012-2013). As Principal investigator

2. Project entitled “Study of in vivo gene expression profiles for identification of determinant(s) involved in neuropathogenicity of enterovirus-71 infection”. Research grant for new scholar, Thailand Research Fund. (2013-2015). As Principal investigator

3. Project entitled “Development of differential immunodiagnosis of hand, foot and mouth disease caused by enterovirus 71 (EV71) and coxsackievirus A16 (CA16) infections by antigen detection method”. Research fiscal year grant, Thammasat University (2013-2015). As Principal investigator

4. Project entitled “Localization and epitope mapping of monoclonal antibodies specific to Vibrio palahaemolyticus” Research fiscal year grant, Thammasat University through Assis. Prof. Dr. Pongsri Tongtawe (2013-2014). As co-investigator

5. Project entitled “Antimicrobial resistant genotypes and lineages of Mycobacterium tuberculosis Thai isolates collected during 2008-2011” Research fiscal year grant, Thammasat University through Assis. Prof. Dr. Potjanee Srimanote (2013-2016). As co-investigator

6. Project entitled “Translational Research on Acute and Chronic Infections and Allergy”. NSTDA Chair Research Grant through Prof. Dr. Wanpen Chaicumpa (2013-2018). As co-investigator

7.  Project entitled “Production of recombinant monoclonal antibody specific to nonstructural proteins of enterovirus 71 for using as biological tool to study pathogenesis of severe hand, foot and mouth disease”. Research fiscal year grant, Thammasat University (2016-2018). As Principal investigator

8. Project entitled “Genotypic characteristics of Streptococcus suis isolated from diseased pig in Central plane, Thailand and human zoonotic transmission potential” Research fiscal year grant, Thammasat University through Assis. Prof. Dr. Potjanee Srimanote (2016-2018). As co-investigator

9. Project entitled “Antibody library construction and the development of antibody array for detection and identification of Vibrio spp.” Research grant, Health System Research Institute through Assis. Prof. Dr. Pongsri Tongtawe (2016-2018). As co-investigator

10.  Project entitled “Development of monoclonal antibodies for Diego blood group typing”. Research fiscal year grant, Thammasat University (2017-2019) through Prof. Dr. Oythip Na Thalang.  As co-investigator

11. Project entitled “Investigation of roles of enterovirus 71 non-structural 3A protein in neuronal cells”. Research fiscal year grant, Thammasat University (2017-2018). As Principal investigator

Research Interests

  1. Development of a prototype of the diagnostic kit for infectious diseases by employing antibody phage display technology
  2. Elucidation of the pathogenic mechanism of hand-foot-mouth disease caused by enterovirus 71 (EV71) and host immune responses
  3. Development of a vaccine for the hand-foot-mouth disease  and screening antivirals

Specializations

Immunology / Virology / Molecular biology / Antibody technology

Other

Education
– B.Sc. (Med. Tech) (Second class honours), Mahidol University, Thailand 2001-2004,
– Ph.D. (Immunology) (International Program), Mahidol University, Thailand, 2008-2010
Thesis entitled “Therapeutic Antibodies For Disease Caused by Biological Weapon (s)”
Received Ph.D. Golden Jubilee grant from Thailand Research Fund through
Prof. Dr. Wanpen Chaicumpa as major advisor

ACADEMIC TRAINING
1. Research trainee at Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan (from May 1st, 2010 to June 30th, 2010): “Cloning and expression of heavy chain of type A botulinum neurotoxin”
2. Research trainee at Hepatitis C Virus Research Laboratory, The University of Adelaide, Australia (from October 17st, 2010 to January 17th, 2011): “Study of human gene candidates involving suppression of HCV replication”
3.Postdoctoral researcher (Professor Emeritus Dr. Wanpen Chaicumpa as mentor); Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
(From 2011 to 2012): Production of therapeutic antibody for HCV infection by phage display technology.

ORAL PRESENTATION
• “Nanobody: A mini-antibody that fights against a bio-weapon” at The XIth RGJ congress, The Thailand Research Fund, April
1st-3rd, 2010, Thailand. (International)
• “Therapeutic nanobody for HCV infection” at Molecular medicine conference 2012, December 19th-22nd, 2012, Thailand. (International)
• “Selection of Antigenic Epitope for Development of Differential Immunodiagnosis of Enterovirus 71” at The 1st International Allied Health Sciences Conference 2014: On the Occasion of 80th Anniversary of Thammasat University, November 4th- 6th, 2014, Thailand
• “ Selection of Murine Single-chain Variable Fragment (MuscFv) Monoclonal Antibodies Specific to EV71 Antigenic Peptides” at Joint Symposium of Universiti Kebangsaan Malaysia and Thammasat University, May 27-28, 2015, Malaysia

AWARDS
1. Outstanding oral presentation award, The XIth RGJ congress, The Thailand Research Fund, April 1st-3rd, 2010, Thailand.
2. The Distinguished Ph.D. Thesis Awards in Health Science, Year 2011. Mahidol University, Thailand. (2012).
3. The Best Ph.D. Thesis Awards in Health Science, Year 2012. National Research Council of Thailand.
4. The Young scientist Year 2014 of Faculty of Allied Health Sciences. Thammasat University.

PUBLICATION
1. Maneewatch S, Thanongsaksrikul J, Songserm T, Thueng-In K, Kulkeaw K, Thathaisong U, Srimanote P, Tongtawe P, Tapchaisri P, Chaicumpa W. Human single-chain antibodies that neutralize homologous and heterologous strains and clades of influenza A virus subtype H5N1. Antivir Ther. 2009; 14: 221-30.
2. Thanongsaksrikul J, Srimanote P, Maneewatch S, Choowongkomon K, Tapchaisri P, Makino S, Kurazono H, Chaicumpa W. A VHH that neutralizes the zinc metalloproteinase activity of botulinum neurotoxin type A. J Biol Chem. 2010; 285: 9657-66
3. Thanongsaksrikul J, Chaicumpa W. Botulinum neurotoxins and botulism: a novel therapeutic approach. Toxins (Basel). 2011; 3 :469-88.
4. Chulanetra M, Sookrung N, Srimanote P, Indrawattana N, Thanongsaksrikul J, Sakolvaree Y, Chongsa-Nguan M, Kurazono H, Chaicumpa W. Toxic marine puffer fish in Thailand seas and tetrodotoxin they contained. Toxins (Basel). 2011; 3:1249-62.
5. Chavanayarn C, Thanongsaksrikul J, Thueng-In K,  Bangphoomi K, Sookrung N, Chaicumpa W. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel). 2012; 4: 554-67.
6. Thueng-in K, Thanongsaksrikul J, Srimanote P, Bangphoomi K, Poungpair O, Maneewatch S, Choowongkomon K, Chaicumpa W. Cell penetrable humanized-VH/VHH that inhibit RNA dependent RNA polymerase (NS5B) of HCV. PLoS One. 2012; 7: e49254.
7. Chulanetra M, Bangphoomi K, Sookrung N, Thanongsaksrikul J, Srimanote P, Sakolvarvaree Y, Choowongkomon K, Chaicumpa W. Human ScFv that block sodium ion channel activity of tetrodotoxin. Toxicon. 2012; 59: 272-82.
8. Pissawong T, Maneewatch S, Thueng-In K, Srimanote P, Dong-din-on F, Thanongsaksrikul J, Songserm T, Tongtawe P, Bangphoomi K, Chaicumpa W. Human monoclonal ScFv that bind to different functional domains of M2 and inhibit H5N1 influenza virus replication. Virol J. 2013; 10: 148.
9. Phalaphol A, Thueng-In K, Thanongsaksrikul J, Poungpair O, Bangphoomi K, Sookrung N, Srimanote P, Chaicumpa W. Humanized-VH/VHH that inhibit HCV replication by interfering with the virus helicase activity. J Virol Methods. 2013; 194: 289-99.
10. Danpaiboon W, Reamtong O, Sookrung N, Seesuay W, Sakolvaree Y, Thanongsaksrikul J, Dong-din-on F, Srimanote P, Thueng-in K, Chaicumpa W. Ophiophagus hannah venom: proteome, components bound by Naja kaouthia antivenin and neutralization by N. kaouthia neurotoxin-specific human ScFv. Toxins (Basel). 2014; 6: 1526-58.
11. Maneewatch S, Adisakwattana P, Chaisri U, Saengjaruk P, Srimanote P, Thanongsaksrikul J, Sakolvaree Y, Poungpan P, Chaicumpa W. Therapeutic epitopes of Leptospira LipL32 protein and their characteristics. Protein Eng Des Sel. 2014; 27: 135-44.
12. Thanongsaksrikul J, Tongtawe P, Srimanote P. Hand, Foot and Mouth Disease: Viruses Causing Disease and Laboratory Diagnosis. J Med Tech Assoc Thailand 2014, Vol. 42 No. 1,: 4767-77.
13. Thueng-in K, Thanongsaksrikul J, Jittavisutthikul S, Seesuay W, Chulanetra M, Sakolvaree Y, Srimanote P, Chaicumpa W. Interference of HCV replication by cell penetrable human monoclonal scFv specific to NS5B polymerase. MAbs. 2014; 6: 1327-39.
14. Dong-din-on F, Songserm T, Pissawong T, Srimanote P, Thanongsaksrikul J, Thueng-in K, Moonjit P, Lertwatcharasarakul P, Seesuay W, Chaicumpa W. Cell penetrable human scFv specific to middle domain of matrix protein-1 protects mice from lethal influenza. Viruses. 2015; 7: 154-79.
15. Glab-Ampai K, Malik AA, Chulanetra M, Thanongsaksrikul J, Thueng-In K, Srimanote P, Tongtawe P, Chaicumpa W. Inhibition of HCV replication by humanized-single domain transbodies to NS4B. Biochem Biophys Res Commun. 2016; 476: 654-64.
16. Glab-Ampai K, Chulanetra M, Malik AA, Juntadech T, Thanongsaksrikul J, Srimanote P, Thueng-In K, Sookrung N, Tongtawe P, Chaicumpa W. Human single chain-transbodies that bound to domain-I of non-structural protein 5A (NS5A) of hepatitis C virus. Sci Rep. 2017; 7: 15042. doi: 10.1038/s41598-017-14886-9.
17. Yin Moe Hlaing, Pongsri Tongtawe, Pramuan Tapchaisri, Jeeraphong Thanongsaksrikul, Unchana Thawornwan, Buppa Archanachan, Potjanee Srimanote. Mutations in streptomycin resistance and their relationship to streptomycin resistance and lineage of Mycobacterium tuberculosis Thai isolates. Tuberculosis and Respiratory Diseases. 2017; 80: 159-168.
18.         Thanongsaksrikul J, Srimanote P, Tongtawe P, Glab-Ampai K, Malik AA, Supasorn O, Chiawwit P, Poovorawan Y, Chaicumpa W. Identification and production of mouse scFv to specific epitope of enterovirus-71 virion protein-2 (VP2). Arch Virol. 2018; doi: 10.1007/s00705-018-3731-z.

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